No mutations, amplifications, deletions, or rearrangements were identified in this cohort of 30 LGNET in the following genes known to be important in the pathogenesis of glial and glioneuronal tumors: ATRX, TERT (including promoter region), TP53, PPM1D, CDKN2A, CDK4, CDK6, RB1, BRAF, KRAS, MAP2K1, PRKCA, PDGFRA, EGFR, MET, NTRK1-3, FGFR2, FGFR3, ALK, ROS1, IDH1, IDH2, H3F3A, H3F3B, HIST1H3B, HIST1H3C, CIC, FUBP1, BCOR, BCORL1, MYB, and MYBL1. The gene discussed is RB1; the disease is mixed neuronal-glial tumor.