Induction of obesity by high-fat diet feeding resulted in a significant increase in the total expression of SPTLC1 (+25.9%, p < 0.05; Figure 2A) in the red gastrocnemius muscle of the HFD group compared to the rats fed a standard chow, which was further declined by two-week CBD injections (−18.2%, p < 0.05; Figure 2A vs. HFD group). The gene discussed is SPTLC1; the disease is obesity due to melanocortin 4 receptor deficiency.