When NK cells cultured in these various media were exposed to GBM cells, the lack of observed change in percentage of TIM-3+ NK cells (Figure 3C) accompanied by a downregulation in TIM-3 expression of 2939.9 Intensity Units was only seen on NK cells cultured in OpTmizerTM medium (Figure 3D), while all RPMI variants showed consistent TIM-3 expression on NK cells after exposure to cancer targets. This evidence concerns the gene HAVCR2 and glioblastoma.