Similar to what was observed with the ALAL-1 CRISPR clones, depletion of ALAL-1 by RNAi reduced the proliferative and colony formation capacity of HCC95 cells while causing more cell death (Fig. S2, E–G), as well as impaired in vivo tumor formation capacity (Fig. 3, L and M). This evidence concerns the gene IKBKB-DT and neoplasm.