USP22 and neoplasm: To further explore the effects of miR-4490 on tumor growth in vivo,BGC-823 cells stably expressing LV-m-NC, LV-miR-4490, LV-vector, LV-USP22, orLV-miR-4490/USP22 were inoculated subcutaneously into nude mice, after which thegrowth of resultant primary tumors was monitored (Fig. 5a).