Using qRT-PCR, we found thatmiR-4490 cells exhibited an increased E-cadherin expression compared to that inm-NC cells, whereas USP22 cells exhibited a decreased E-cadherin expressioncompared to that in USP22/miR-4490 co-expressing cells and empty vectortransfected cells (Fig. 5g).Additionally, the presence of GC metastases in the lungs was confirmed by IHCusing anti-E-cadherin and anti-vimentin antibodies (Fig. 5h). Here, USP22 is linked to gastric cancer.