To verify the transcriptional levels of the genes identified in the transcriptome analysis to be important in the mechanism by which sIL-13Rα2-Fc protects against rat intervertebral disc degeneration and to confirm the accuracy of the bioinformatics analysis, we used real-time fluorescence quantitative PCR to verify the expression of the candidate genes Runx1, Sod2, Tnfaip6, Aldh3a1, Galnt3, Fgf1, Celsr1, and Adamts8. The results are shown in Figure 5. This evidence concerns the gene FGF1 and Intervertebral disk degeneration.