ADAMTS8 and Intervertebral disk degeneration: To verify the transcriptional levels of the genes identified in the transcriptome analysis to be important in the mechanism by which sIL-13Rα2-Fc protects against rat intervertebral disc degeneration and to confirm the accuracy of the bioinformatics analysis, we used real-time fluorescence quantitative PCR to verify the expression of the candidate genes Runx1, Sod2, Tnfaip6, Aldh3a1, Galnt3, Fgf1, Celsr1, and Adamts8. The results are shown in Figure 5.