Intraperitoneal (i.p.)injection of high-dose c-di-GMP directly activated caspase-3 and triggered 4 T1 tumor cell apoptosis in vitro; 15 nmol of c-di-GMP reduced the growth of 4 T1 tumor cells in vitro by 70% and 150 nm reduced it by 92%, while low-dose c-di-GMP (0.01-2 nmol) accelerated the adaptive T cell response by converting a subgroup of myeloid-derived suppressor cells (MDSCs) into immune stimulatory cells producing IL-12 [105]. Here, CASP3 is linked to neoplasm.