Compared with sham rats, 5/6NX rats showed significantly increased levels of SCr, BUN, and HA, as well as increased fibrosis area, glomerular sclerosis index, and fibrotic marker levels (COL1A1, VIM, and ACTA2), suggesting that the 5/6NX rat model was effective. This evidence concerns the gene COL1A1 and glomerulosclerosis.