Compared with sham rats, 5/6NX rats showed significantly increased levels of SCr, BUN, and HA, as well as increased fibrosis area, glomerular sclerosis index, and fibrotic marker levels (COL1A1, VIM, and ACTA2), suggesting that the 5/6NX rat model was effective. The gene discussed is ACTA2; the disease is Glomerular sclerosis.