Furthermore, Western blot analysis results revealed significantly upregulated pro-caspase-1/caspase-1 and pro-IL-1β/IL-1β in lung tissues of LPS-induced mice, which was indicative of successful ALI model establishment, accompanied by activation of NLRP3 inflammasome along with promoted maturation and release of cytokines. Here, NLRP3 is linked to acute respiratory distress syndrome.