As with HIV‐1 GFP produced in the presence of PIs, infection of PMA‐treated THP‐1 shSAMHD1 cells with the HIV‐1 GFP ∆CA‐SP1 mutants led to a ∆CA‐SP1 dose‐dependent increase in the expression of CXCL‐10 (Fig 2C) and MxA mRNA (Fig 2D), and CXCL‐10 at the protein level (Fig 2E). This evidence concerns the gene MX1 and infection.