SP1 and infection: We conclude that viral DNA is the active PAMP and this notion was also supported by the observation that mutation D185E in the RT active site (HIV‐1 ∆CA‐SP1 RT D185E) also reduced activation of IFIT‐1 luc expression (Fig 3E) and CXCL10 secretion (Fig 3F) on infection of the THP‐1 IFIT‐1 reporter cells.