Because ITDs most frequently occur in the FLT3 gene of patients with AML, we first sought to compare ScanITD with 3 existing ITD detectors: ITDseek v1.2 [11], Genomon-ITDetector [26], and Pindel v0.2.5 [8], using 2 simulation datasets of hotspot FLT3 ITDs at 275 and 100 bp paired-end reads. This evidence concerns the gene FLT3 and acute myeloid leukemia.