Western blot was used to detect the phosphorylated forms of MAPK family members (ERK1/2, JNK and p38 MAPK), AKT and JAK‐STAT family members (JAK1 and STAT1) in glioma cells which transfected with NC and KLHDC8A siRNA. The gene discussed is KLHDC8A; the disease is central nervous system cancer.