The direct RT-PCR data showed that the mRNA levels of β-actin (as internal control) were not changed with the treatment of phloretin, while the total levels of 5′-UTR of Sp1 mRNA (as input) were decreased with the treatment of phloretin in a concentration-dependent manner in both PCa cell lines (Figure 1(d)). This evidence concerns the gene SP1 and posterior cortical atrophy.