DDHD1 and infection: To exclude the possibility of off-target effects and to determine whether the phospholipase activity of DDHD1 plays a role in neurite outgrowth, siRNA-resistant mCherry-DDHD1 wild-type and enzymatic inactive mCherry-DDHD1S537A, in which catalytic residue Ser537 was replaced by Ala, were expressed by infection with recombinant viruses encoding the proteins (Baba et al., 2014).