Consistent with our previous assay, SAMHD1 level was reduced at 72 and 96 h after infection at an MOI of 1; however, it was almost completely blocked when cells were treated with PAA at 24 h after infection, demonstrating that SAMHD1 loss requires viral DNA replication and/or viral late gene expression (Figure 2D). The gene discussed is SAMHD1; the disease is infection.