To examine whether CMV infection impacts TLR-driven host responses, we compared the secretion of pro-inflammatory cytokines from the whole blood of 16 CMV mononucleosis patients and 18 controls stimulated with TLR2, TLR3, TLR4, TLR7, TLR7/TLR8, and TLR9 specific agonists. Here, TLR9 is linked to cytomegalovirus infection.