To further verify the involvement of TGF-β1 in the regulation of PI3K/AKT pathway, we generated a TGF-β1-depletion HCT-116 cell line by lentivirus infection (Figure 8A), our western blot result showed that the expression of TGF-β1 was significantly downregulated after infection (Figure 8B). Here, TGFB1 is linked to lentivirus infection.