EZH2 and mantle cell lymphoma: To understand the mechanism of EZH2 mediated regulation of cdkn2b, we performed chromatin immunoprecipitation (ChIP) assays on MCL cells cultured in absence or presence of GSK126 using the anti-H3K27me3 antibody or IgG control, and the primers against cdkn2b promoter as depicted in Figure 5C.