To explore further the relationship between LARGE and lymph node metastasis in prostate cancer, we transduced the cells with oe-DLX6-AS1 and/or oe-LARGE, which showed that overexpression of DLX6-AS1 enhanced the expression of MMP-9, uPAR, and cathepsin B, while LARGE overexpression repressed their expression. The gene discussed is CTSB; the disease is prostate cancer.