Cytokine analyses of MDA-MB-231-ERK5-ko and Hs-578T-ERK5-ko cells compared to parental controls showed that ERK5-ko resulted in upregulation of ADIPOQ and downregulation of IL-8, OPN, TARC, IGFBP3, and MPO showing a role for ERK5 in regulation of inflammatory signaling pathways in the tumor microenvironment (Supplementary Figure 9). This evidence concerns the gene CXCL8 and neoplasm.