Some genes that were downregulated in MDA-MB-231-ERK5-ko cells were not downregulated in Hs-578T-ERK5-ko, including LOX. Evaluation of the RNA-seq data also demonstrated alterations of inflammatory genes associated with the extracellular tumor environment (IL-8, IGFBP3, ADIPOQ) in the knockout cell lines. Here, IGFBP3 is linked to neoplasm.