Meanwhile, our study showed that positive rates of DSG1 and DSG2 expression were significantly lower in the case of poorly differentiated types, TNM stages III or IV, positivity of lymph node metastasis, regional invasion, and no resection (biopsy only) (P < 0.01), suggesting that DSG1 and DSG2 may be involved in tumorigenesis of EHCC. Here, DSG2 is linked to metastatic malignant neoplasm in the lymph nodes.