We used a neuroblastoma PDX model where tumors were dissected into 1-mm3 pieces and cultured in duplicate on a presoaked gelatin sponge in 24-well plates containing 500 μL RPMI 1640 with 10% FBS, antibiotic/antimycotic solution, 0.01 mg/mL hydrocortisone, and 0.01 mg/mL insulin. Here, INS is linked to neuroblastoma.