By using the elastic net and RRA analysis, we screened nine gene expression signatures, including AFM, AKR1C3, CYP1A2, CYP2E1, GPC3, HAMP, HBB, MT1G, and SPINK1, to construct a diagnosis prediction model for early HCC (DP.eHCC). This evidence concerns the gene SPINK1 and hepatocellular carcinoma.