By constructing a PPI network, we found that DUXAP8 could regulate multiple cell cycle regulators in HCC, including BUB1, CDCA5, CDCA8, SPDL1, NEDD1, NCAPD2, SGO1, TPX2, CENPE, KNSTRN, and NDE1. Here, NEDD1 is linked to hepatocellular carcinoma.