In order to investigate the mechanisms of DUXAP8 in HCC, we conducted co-expression and bioinformatics analysis of DUXAP8, and the results showed that DUXAP8 was involved in regulating multiple cell cycle-related biological processes, such as the cell cycle, the mitotic cell cycle, nuclear division, chromosome segregation, and cell division. The gene discussed is DUXAP8; the disease is hepatocellular carcinoma.