By constructing a PPI network, we found that DUXAP8 could regulate multiple cell cycle regulators in HCC, including BUB1, CDCA5, CDCA8, SPDL1, NEDD1, NCAPD2, SGO1, TPX2, CENPE, KNSTRN, and NDE1. The gene discussed is CDCA5; the disease is hepatocellular carcinoma.