PLCB3-S845L results in a loss-of-function variant, where defective intracellular Ca2+ redistribution and PKCs' activation limited the IL-8, IL-1β, and MUC5 expression in CF patient-derived airway epithelial cells exposed to P. aeruginosa or CF patient-derived mucopurulent material. Here, CXCL8 is linked to cystic fibrosis.