However, although TMEM16A activity was reported to negatively regulate pro-inflammatory cytokine, including IL-8, synthesis in human CF bronchial epithelia (Veit et al., 2012), TMEM16A is a positive regulator of endothelial ROS (Ma et al., 2017), and its inhibition would lead to decreased ROS in endothelial cells, and not the increase reported in the current study, supporting the involvement of CFTR in the observed responses to GlyH-101. Here, ANO1 is linked to cystic fibrosis.