EPHA2 and nasopharyngeal carcinoma: Soft agar colony formation assay showed that EphA2-WT dramatically increased the anchorage-independent growth of NPC cells, whereas EphA2-YA failed to do it as compared to endogenous EphA2 knockdown (Fig. 1e), indicating that Y772A mutation abolished the effects of EphA2-WT on the anchorage-independent growth of NPC cells.