MARCO and glioblastoma: Additionally, multi-color immunohistochemistry (IHC) analyses of human GBM specimens demonstrated prevalence of putative MA-TAM master regulators and their target genes, including MARCO, in mesenchymal-classified tumors, further confirming the authenticity and feasibility of our systematic process to identify MA-TAM transcriptional networks (Fig. 1d and Additional file 1: Figure S4).