GJB2 and hearing loss disorder: If we focus on the most prevalent variants causing hearing loss in the Roma population, there are only two other variants known so far, the c.71G>A (p.W24*) in GJB2 and the c.1331+2T>C in MARVELD2. The detected carrier frequency 3.8% for c.2158-2A>G in MANBA is very similar to the average carrier rate of c.71G>A (p.W24*) in GJB2 among the European Roma, which is in the 4–5% range [31].