Here, we found that CXCR5 siRNA and CXCR5 antibody markedly inhibited CXCL13-induced VCAM-1 expression and cell migration, suggesting that the CXCL13/CXCR5 interaction regulates VCAM-1-mediated migration of human osteosarcoma cells. This evidence concerns the gene VCAM1 and osteosarcoma.