In order to independently study whether there is a correlation between a reduction of TPC2 expression and the enhanced expression of YAP/TAZ target genes, we used RNA interference (RNAi) to transiently silence TPC2 expression for 24 h in the CHL1 and MeWo metastatic melanoma cell lines (Figure 4B). The gene discussed is TPCN2; the disease is metastatic melanoma.