In order to characterize whether EGFR was activated during infection of A549 cells, protein levels of phospho-EGFR at Tyr1068 (Y1068), a known inducible autophosphorylation site correlated with EGFR kinase activity, was detected via Western blot at 24, 36, and 48 hpi, as determined elsewhere [15]. The gene discussed is EGFR; the disease is infection.