Our data showed that expressions of afamin, alpha-2-HS-glycoprotein, apolipoprotein B-100, clusterin, hepatocyte growth factor-like protein, and kininogen-1 were stimulated in HCC but repressed in CCA, while the expression of Ig lambda chain V region 4A was stimulated in CCA but repressed in HCC (Fig 2B). Here, AFM is linked to cholangiocarcinoma.