In the example in Figure 1, we integrated four scRNA-seq datasets of mouse T cells from public repositories, composed of (i) CD8+ tumor-infiltrating lymphocytes (TILs) (Carmona et al., 2020); (ii) CD4+ and CD8+ TILs (Xiong et al., 2019); (iii) CD4+ T cells from tumors (Magen et al., 2019) and (iv) CD4+ T cells from tumor-draining lymph nodes (dLN) (Magen et al., 2019). The gene discussed is CD8A; the disease is neoplasm.