We identified a median of three additional mutations per FLT3-ITDmut AML (range, 1–7), with N-RAS (5 of 12, 41%), RUNX1 (4 of 12, 33%) and DNMT3A (3 of 12, 25%) as the most frequent alterations. This evidence concerns the gene RUNX1 and acute myeloid leukemia.