VCL and infection: Given the profound effect of infection and TarP ectopic expression on FA stability, we investigated using iPALM their effects on FA organization as described under “Materials and methods.” The localization of paxillin, FAK, and vinculin, all fused to mTurquoise2, was monitored in mock-infected, CtrL2-infected, TarP ΔPRD, or LDVBD-transfected cells (Fig. 7).