However, TFE3 IHC can show false-positive results, TFE3 IHC combined with other diagnosis tools, such as break-part TFE3 fluorescence in situ hybridization (FISH), Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and cytogenetic analysis can be effective means to diagnose the Xp11.2 tRCC. The gene discussed is TFE3; the disease is renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.