Hypermethylation of MLH1, RASSF1A/2A, PTEN, and APC has been reported in EEC and less frequently in SEC [11,12,13,14], which may be a consequence of the increased expression of DNA methyltransferases DNMT1 and DNMT3B in EEC [15]. This evidence concerns the gene MLH1 and exstrophy-epispadias complex.