Validation of the RNA-sequencing for specific AF-associated genes in a second murine cohort demonstrated dysregulation of genes encoding K+ channel subunits comprising Ito (Kcnip2, 0.37 ± 0.11, P < 0.001), IKr (potassium voltage-gated channel subfamily H member 2, Kcnh2, 1.4 ± 0.067, P < 0.001), and IKs (potassium voltage-gated channel subfamily Q member 1, Kcnq1, 2.3 ± 0.064, P < 0.000001) (Figure 4C, Supplemental Table 14). This evidence concerns the gene KCNH2 and atrial fibrillation.