Sequencing the 5’-flanking region of TNC revealed several potential binding sites for multiple transcription factors.68 Reporter activation assay in fibroblasts or cancer cell lines revealed multiple transcription factors regulating TNC gene expression, such as MKL1, OTX2, SP1, JUN, et al.69–72 Among all these transcription factors, NF-κB was reported to activate TNC expression in rat cardiac myocytes by mechanical deformation.73 It would be interesting to explore how necroptosis triggers the transcription of TNC or other ECM proteins during tissue repair. The gene discussed is OTX2; the disease is cancer.