Although our analysis did not show association of CA9 and PD-L1 in the meningioma cohort (One way ANOVA test, F-ratio = 2.9, p = 0.09), we found 46% (26/57) positive CA9 expression in primary meningioma with significant positive relationship with high PD-L1 expression (One way ANOVA test, F-ratio = 5.3, p = 0.025) (Fig. 2H). This evidence concerns the gene CA9 and meningioma.