To create a population of trackable donor CD8 cells reactive against a miHA expressed by leukemia cells, we vaccinated C3H.SW (H-2b) or B6 (H-2b) mice against the Kb-restricted mouse miHA H6019 using an antibody against DEC205 which was modified to express the H60 epitope LTFNYRNL (DEC-H60) with an agonist antibody against CD40 (FGK45)18. The gene discussed is CD8A; the disease is leukemia.