To study the involvement of ERRβ in the MLN4924-mediated E-Cadherin upregulation, we performed qRT-PCR on MLN4924-treated MDA-MB-231 and MCF7 breast cancer cells with and without ERRβ knockdown and found that the knockdown of ERRβ significantly reduced E-cadherin mRNA levels even after MLN4924 treatment (Fig. 7c). This evidence concerns the gene CDH1 and breast cancer.