AKT1 and non-small cell lung carcinoma: In addition, NSCLC cells were treated with the PI3K/Akt inhibitor LY294002 or LY294002 + miR-425-5p mimics mimic (Fig. 5b), Kinase activity assays further showed that PI3K activity in NSCLC transfected with LY294002 was significantly lower than that transfected with mimic control (P < 0.01), and PI3K activity in NSCLC cells transfected with both LY294002 and miR-425-5p mimic was significantly higher than that transfected with LY294002 (P < 0.01).