In the study of clinical samples of human gastric cancer, Rosivatz et al. found that the down-regulation of E-cad expression was closely related to the up-regulation of transcription factors Snail, Twist, and SIP1 [22], while Wang et al. also found high expression of transcription factors such as Snail, Twist and Slug in immortalized gastric epithelial cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901, which were negative for E-cad expression [23]. This evidence concerns the gene TWIST1 and gastric cancer.