In the present study, NF-κB activation and up-regulation of TGF-β1 and TNF-α were triggered as a result of NASH induced by an HFD combined with DMN injections in Cx32ΔTg rats, even though these changes were smaller in the liver of Wt rats. This evidence concerns the gene NFKB1 and metabolic dysfunction-associated steatohepatitis.