The prevalence of HIV in Eswatini (formerly Swaziland) is amongst the highest in the world.7 Despite the high HIV prevalence and known risk of cryptococcal meningitis, CrAg prevalence amongst people with advanced HIV disease, or CD4 < 200 cells/mm3, is unknown in Eswatini. The gene discussed is CD4; the disease is Cryptococcal meningitis.