In addition, we found that the CAFs not only had markedly reduced expression of CAFs markers, including α‐SMA and FAP, but also exhibited significantly weaker migrative potential, when they were cultured with the CM from imperatorin‐treated ESCC cells compared with those exposed to control CM (Figure 4F,G), and this could be rescued when TGFβ2 recombination protein were added to the CM derived from imperatorin‐treated ESCC cells. Here, ACTA1 is linked to esophageal squamous cell carcinoma.