That was higher than the diagnostic performance of the anti-citrullinated peptide antibody (ACPA) assay with a sensitivity of 83.43% and specificity of 86.67% (AUC = 0.875) and rheumatoid factor (RF) assay with a sensitivity of 80.9% and specificity of 46.67% (AUC = 0.720) in diagnosing RA. This evidence concerns the gene PRTN3 and rheumatoid arthritis.