As expected, we found that lentivirus-mediated overexpression of PTEN enhanced mitochondrial respiration of HCC cells, as evidenced by enhanced basal OCR, ATP-linked respiration, maximal OCR, spare respiratory capacity in HHCC cells with lentiviral transduction of oe-PTEN compared with vector-NC (Fig. 4b). The gene discussed is PTEN; the disease is hepatocellular carcinoma.