We found that lentivirus-mediated overexpression of PTEN reduced the glycolysis rate of HCC cells, as evidenced by reduced ECAR under basal glycolysis, lower percentage of ECAR from basal glycolysis, and reduced ECAR of compensatory glycolysis in HHCC cells with lentiviral transduction of oe-PTEN compared with vector-NC (Fig. 4a). This evidence concerns the gene PTEN and hepatocellular carcinoma.